DPI激活GPR3-b-Arrestin2-PKM2增强kupffer细胞的糖酵解[单细胞RNA-seq]

Single-cell RNAseq analysis of liver cells from NASH and cirrhosis patients has identified the presence of TREM2+ disease-associated macrophages (DAMs) in the liver that display lower expression of metabolic genes. To determine whether the DAMs are also present in patient with mild NFALD, we performed scRNAseq of freshly isolated immune cells from liver biopsies of 3 healthy donors and 3 mild NFALD patients. Kupffer cells were specifically focused.

针对非酒精性脂肪性肝炎（NASH）与肝硬化患者的肝脏细胞开展的单细胞RNA测序分析，已在肝脏中鉴定出TREM2阳性的疾病相关巨噬细胞（DAMs），这类细胞的代谢基因表达水平较低。为明确此类疾病相关巨噬细胞是否同样存在于轻度非酒精性脂肪性肝病（NFALD）患者体内，我们对3名健康供者与3名轻度非酒精性脂肪性肝病患者的肝脏活检组织中新鲜分离的免疫细胞进行了单细胞RNA测序（scRNAseq），并重点聚焦于库普弗细胞（Kupffer cells）。