DPI激活GPR3-b-Arrestin2-PKM2增强kupffer细胞的糖酵解[单细胞RNA-seq]

Single-cell RNAseq analysis of liver cells from NASH and cirrhosis patients has identified the presence of TREM2+ disease-associated macrophages (DAMs) in the liver that display lower expression of metabolic genes. To determine whether the DAMs are also present in patient with mild NFALD, we performed scRNAseq of freshly isolated immune cells from liver biopsies of 3 healthy donors and 3 mild NFALD patients. Kupffer cells were specifically focused.

针对非酒精性脂肪性肝炎（NASH）与肝硬化患者的肝脏细胞开展的单细胞RNA测序分析，已在肝脏中鉴定出TREM2阳性的疾病相关巨噬细胞（DAMs），这类细胞的代谢基因表达水平较低。为明确此类疾病相关巨噬细胞是否同样存在于轻度非酒精性脂肪性肝病（NFALD）患者体内，我们对3名健康供者与3名轻度非酒精性脂肪性肝病患者的肝脏活检组织中新鲜分离的免疫细胞进行了单细胞RNA测序（scRNAseq），并重点聚焦于库普弗细胞（Kupffer cells）。

搜集汇总

背景与挑战

背景概述

该数据集通过单细胞RNA测序技术，研究了DPI激活GPR3-b-Arrestin2-PKM2通路如何增强Kupffer细胞的糖酵解过程，重点关注非酒精性脂肪肝病（NAFLD）的病理机制。数据来源于人类肝脏免疫细胞，包括健康捐赠者和轻度NAFLD患者的样本，使用Illumina NovaSeq 6000平台进行转录组测序，旨在揭示疾病相关巨噬细胞的代谢变化。