Supplementary dataset for "Characterization of Human Stem Cell-Derived Hepatic Stellate Cells and Liver Sinusoidal Endothelial Cells During Extended in vitro Culture"

The dataset contains confocal immunofluorescence images of human pluripotent stem cell-derived hepatic stellate cells (scHSCs) and human primary HSCs (pHSCs). In the homeostatic liver, HSCs exist in a quiescent state. Upon liver injury, HSCs differentiate into an activated, myofibroblast-like state. The activated HSCs are phenotypically characterized by the intracellular expression of alpha-smooth muscle actin (a-SMA). Transforming growth factor beta (TGF-b) is a potent HSC activator and is commonly used to assess the activation capacity of cultured HSCs. In this dataset, scHSCs and pHSCs were grown in vitro and stained with immunofluorescent labels for the HSC marker PDGFR-b as well as the activation-related proteins a-SMA and COL1a1. Image analysis of the dataset was performed to quantify a-SMA fluorescence intensity to assess the activation status of the HSCs in the presence and absence of TGF-b. An example script to analyze the data is provided on GitHub: https://github.com/ingridwilhelmsen/a-SMA_analysis/blob/main/a-SMA_analysis-Activation.ipynb

该数据集收录了源自人源多能干细胞的人肝星状细胞（scHSCs）和人体原代肝星状细胞（pHSCs）的共聚焦免疫荧光图像。在肝脏稳态中，肝星状细胞处于静息状态。在肝脏损伤的情况下，肝星状细胞分化为激活的肌成纤维细胞样状态。激活的肝星状细胞在表型上以细胞内α-平滑肌肌动蛋白（α-SMA）的表达为特征。转化生长因子β（TGF-β）是一种有效的肝星状细胞激活剂，常用于评估培养的肝星状细胞的激活能力。在本数据集中，scHSCs和pHSCs在体外培养，并使用免疫荧光标记进行肝星状细胞标记物PDGFR-β以及与激活相关的蛋白α-SMA和COL1a1的染色。对数据集进行了图像分析，以量化α-SMA荧光强度，以评估TGF-β存在与否下肝星状细胞的激活状态。一份分析数据的示例脚本已提供在GitHub上：https://github.com/ingridwilhelmsen/a-SMA_analysis/blob/main/a-SMA_analysis-Activation.ipynb