Characterization of the coupled genes for Pik-h via gain and loss of function approaches.

aT0 plants derived from each construct were inoculated with the Pikh-avirulent isolates, CHL346 or CHL42. R, resistant; MR, moderate resistant; MS, moderate susceptible; S, susceptible.bThe success ratios for gain- and loss-of-function complementation tests were calculated as (R+MR)/(R+MR+MS+S), and (MS+S)/(R+MR+MS+S), respectively.cThe Pik allele-specific RNAi constructs, KP3 RNAi1 and KP4 RNAi, which correspond to Pikp-1 and Pikp-2, respectively [40], were transformed into the Pik-h carrier cv K3.