Table_3_Genetic analysis of potential biomarkers and therapeutic targets in ferroptosis from psoriasis.xls

IntroductionFerroptosis is associated with multiple pathophysiological processes. Inhibition of ferroptosis has received much concern for some diseases. Nonetheless, there is no study comprehensively illustrating functions of ferroptosis-related genes (FRGs) in psoriasis.MethodsIn this study, FRGs together with psoriasis-associated data were obtained in Ferroptosis Database (FerrDb) and gene expression omnibus (GEO) database separately. This work identified altogether 199 psoriasis-associated DE-FRGs, and they were tightly associated with immunity and autophagy modulation. Thereafter, the present study utilized SVM-RFE and LASSO algorithms to identify NR5A2, CISD1, GCLC, PRKAA2, TRIB2, ABCC5, ACSF2, TIMM9, DCAF7, PEBP1, and MDM2 from those 199 DE-FRGs to be marker genes. As revealed by later functional annotation, the marker genes possibly had important effects on psoriasis through being involved in diverse psoriasis pathogenesis-related pathways such as cell cycle, toll-like receptor (TLR), chemokine, and nod-like receptor (NLR) pathways. Moreover, altogether 37 drugs that targeted 11 marker genes were acquired. Besides, based on CIBERSORT analysis, alterations of immune microenvironment in psoriasis cases were possibly associated with PRKAA2, PEBP1, CISD1, and ACSF2.DiscussionTaken together, this work established the diagnostic potency and shed more lights on psoriasis-related mechanism. More investigations are warranted to validate its value in diagnosing psoriasis before it is applied in clinic.

铁死亡与多种病理生理过程密切相关。对于某些疾病而言，抑制铁死亡引起了广泛的关注。然而，至今尚无研究能够全面阐述铁死亡相关基因（FRGs）在银屑病中的功能。研究方法：在本研究中，我们从铁死亡数据库（FerrDb）和基因表达综合数据库（GEO）中分别获取了与银屑病相关的FRGs数据。本研究共确定了199个与银屑病相关的差异表达FRGs，它们与免疫调节和自噬密切相关。随后，本研究利用SVM-RFE和LASSO算法从这199个差异表达FRGs中识别出NR5A2、CISD1、GCLC、PRKAA2、TRIB2、ABCC5、ACSF2、TIMM9、DCAF7、PEBP1和MDM2作为标记基因。后续的功能注释表明，这些标记基因可能通过参与细胞周期、TLR、趋化因子和NLR等与银屑病发病机制相关的多种途径，对银屑病产生重要影响。此外，还获得了针对11个标记基因的37种药物。基于CIBERSORT分析，银屑病患者免疫微环境的改变可能与PRKAA2、PEBP1、CISD1和ACSF2有关。讨论：综合来看，本研究建立了银屑病的诊断潜力，并进一步揭示了银屑病的相关机制。在临床应用之前，有必要进行更多研究以验证其在诊断银屑病中的价值。