Overview of attempts to create ‘hexas’ for TaPLDß1 and TaWRKY11.

Number of progeny screened (F2 progeny) and number of progeny identified with expected tetra and hexa genotypes (frequency of primary mutants is not shown; these were similar to expected for all crosses.‘-’ indicates that a given parent was not used in the cross, or a given deletion was not expected in the progeny. N.B. for TaPLDß1 A-D tetras were crossed with B-D tetras to produce hexas, and thus all progeny were expected to possess a D deletion. Therefore AB-deletion lines (possessing deletions of A and B, but with D intact) were not expected within the progeny.Asterisks indicate significantly different observed frequencies of a deletion genotype compared to expectations;* indicates P ** indicates P ‡ ‘Index’ refers to the expected genotypic ratio within F2 progeny of crosses performed as given in Table E in S1 File.Overview of attempts to create ‘hexas’ for TaPLDß1 and TaWRKY11.