Shinya Inoue, Edward Salmon (2011) CIL:11954, Chaetopterus sp. 1 Woods Hole MA, oocyte. CIL. Dataset

The movie shows the effect of pressure on the spindle of the Chaetopteris egg. 100 Atm. of pressure induces depolymerization of spindle microtubules leading to the loss of birefringence in this polarizing microscopy study. As the spindle loses its birefringence and slowly shrinks, the chromosomes are pulled to the cell surface. When the pressure is returned to 1 Atm., the spindle microtubules reassemble and push the chromosomes away from the cell surface. The movie shows several rounds of pressure increases and decreases in which the birefingence of the spindle is clearly seen with polarizing optics. The last sequence shows the same cycle using phase contrast where the spindle is not so clearly seen.

本视频展示了压力对鳞毛蕨卵子纺锤体的影响。在100个大气压的条件下，纺锤体微管发生去聚合，导致在该偏光显微镜研究中，其双折射性丧失。随着纺锤体双折射性的丧失及其缓慢的收缩，染色体被拉向细胞表面。当压力恢复至1个大气压时，纺锤体微管重新组装，并将染色体从细胞表面推开。视频中展示了多次压力的增加和降低循环，其中通过偏光显微镜可以清晰地观察到纺锤体的双折射性。最后一段序列展示了使用相位对比显微镜进行相同循环的过程，此时纺锤体并不那么清晰可见。