Primary transmission of classical CJD, GSSA117V and vCJD prions to transgenic mice expressing huPrP117V,129V+/+ (117VV Tg31).

IPD = inherited prion disease; IHC = immunohistochemistry; IB = immunoblotting; ND = not determined; GH = growth hormone; DM = dura mater.*According to classification of Hill et al. [53].†Positive either by clinical signs, western blotting and/or immunohistochemistry; primary antibody was either 3F4 or ICSM 35.‡One PBS-inoculated mouse culled at 582 days post-inoculation due to intercurrent illness, had minimal PrP plaques (Figure S1A) in the anterior commissure but was negative by IB.§One mouse was positive by the presence of PrP21–30 kDa on immunoblot (Figure 3B, lane 4) but tissue was unavailable for immunohistochemical analysis.¥Post-inoculation survival period (days): 292, 387, 450, 455, 498, 515 and 547.