Gene expression modulation assessed by quantitative PCR in keratinocytes of reconstructed skins exposed to UVA1.
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Reconstructed skins were exposed to 20 or 40 J/cm2 UVA1 and recovered 2, 6 or 24 hours later. Expression of genes found modulated in the microarray study and/or representative of main functional families was analyzed by quantitative PCR in keratinocytes of reconstructed skin. Ratios of modulation induced by UVA1 exposure were calculated for each condition as the ratio of mean mRNA amount in UVA1 exposed samples to mean mRNA amount in sham exposed control samples (columns 2 to 7). Bold text indicate mean ratio values with significant differences between UVA1 exposed and control samples (p
创建时间:
2015-12-02



