Single-cell RNA sequencing of CD45+ cells resided in the small intestine of wild-type and Dusp6 knockout mice

To assess whether Dusp6 deficiency exerts alteration to intestinal immune cells specifically, we further applied the single-cell RNA sequencing with 10X Genomics platform in Dusp6-knockout (D6KO) mice and littermate wild-type (WT) control mice under chow diet. The intestines of wild-type and Dusp6-knockout mice (11 weeks old) were harvested and the live intestinal immune cells from the intraepithelial compartment and the laminar propria were collected with CD45+ marker by magnetic-activated cell sorting (MACS) MicroBeads and fluorescence-activated cell sorting (FACS) for downstream 10X Genomics Chromium Single Cell 3' Solution. Comparison of intestinal CD45+ cells between wild-type and Dusp6 knockout mice