Dataset for "Gap junctions desynchronize a neural circuit to stabilize insect flight"

Data guide

For each figure, we provide one data folder. Each folder contains one overview Excel file that provides all source data that is shown in the respective figure. For some figure panels additional subfolders with original data files are provided. Please note that the data for figures 3A-J (except F and G) have been uploaded together with the analyses and simulation code and are accessible under the doi: 10.5281/zenodo.7740678

Data Figure 1:

Overview Excel file with all source data: Figure 1.xlsx

Folder Panel A:

Recordings shown in A and G with sorted spikes: figure 1a_original recording.smr

Data matrix of waveforms shown in A as text file: figure 1a_waveforms_200818_561.5-566.5sec.txt

Folder Panels B+F: all original recordings with sorted spikes that have been analyzed for panels B and F:

171106_m1.SMR

181029_m1.smr

190522_m1.SMR

200305_m1.smr

200818_m1.smr

200824_m1.smr

200827_m1.SMR

201005_m1.smr

Folder Panel C: all original recordings as .smr (Spike2) files that went into the analyses shown in figure 1c.

100 recordings in folders “Fly 1 to Fly 100”

1 recording in folder “single animal”

the waveforms of these 101 animals are also as text in file Figure 1.xlsx

Folder Panel D

The original data file of the recording shown in figure 1D as axon file: 20128002_OUT1_firing.abf

The selected waveforms shown in figure 1D are also as text in file Figure 1.xlsx

All recordings that underlie the analysis in figure 1E are as number matrices in the file Figure 1.xlsx

The spike events shown in figure 1G are as number matrices in the file Figure 1.xlsx

Data Figure 2:

Overview Excel file with all source data: Figure 2.xlsx

The number matrices for all traces shown in figure 2A are as number matrices in the file Figure 2.xlsx

Panel B: all number matrices of the event times that underlie the analyses shown in figure 2B:

Folder “control”: .txt of event times of the 7 control animals

Folder “ShakB-RNAi”: .txt of event times of the 7 ShakB-RNAi-kd animals

Folder “ShakB-oe”: .txt of event times of the 7 ShakB-oe (overexpression) animals

Panel C original confocal image stack for image data shown in figure 2C:

original Leica file:  figure 2c_image stack.lif

Folder “tiff” with same image stack saved as tiff

Panel D: The number matrices underlying the waveforms of the traces shown in Figure 2D are contained in the file Figure 2.xlsx

Panel E: The number matrices underlying the waveforms of the traces shown in Figure 2E are contained in the file Figure 2.xlsx

Panel F: The number matrices of all pre- and postsynaptic voltage areas of paired MN recordings and the analyses thereof for Figure 2F (left) are contained in the file Figure 2.xlsx. The number matrices of all pre- and postsynaptic voltage amplitudes of paired MN recordings and the analyses thereof for Figure 2F (right) are contained in the file Figure 2.xlsx

Panel G: The number matrices underlying the waveforms of the traces shown in Figure 2G are contained in the file Figure 2.xlsx

The number matrices underlying the waveforms of the traces shown in Figure 2H are contained in the file Figure 2.xlsx

The number matrices underlying the waveforms of the traces shown in Figure 2I are contained in the file Figure 2.xlsx

Data Figure 3: (all data for Fig. 3 except f and g are at doi: 10.5281/zenodo.7740678)

Overview Excel file with all source data for Figures 3G and F: Figure 3.xlsx

Panel F: The number matrices for all traces shown in figure 3F (command voltage +50mV) and for 14 additional command voltage steps are contained in the file Figure 3.xlsx

Panel F: The number matrices for the waveforms of all traces shown in figure 3F (command voltage +50mV) and for 14 additional command voltage steps are contained in the file Figure 3.xlsx

Panel G: The number matrices for the waveforms of all traces shown in figure 3G are contained in the file Figure 3.xlsx

original data file from which the traces in figure 3G (top) were taken: control MN4_3_0s-87s.SMR

original data file from which the traces in figure 3G (bottom) were taken: UAS shab81 2022_10_07_0s-87s.SMR

Data Figure 4:

Overview Excel file with all source data: Figure 4.xlsx

Panel A: The number matrices for all event signals and all waveform traces shown in figure 4A are as number matrices in the file Figure 4.xlsx

Figure 4B (top):

The number matrices for the waveforms (voltage recording and fit) shown in Figure 4b (top) are contained in the file Figure 4.xlsx

The number matrices for the waveforms of the voltage recordings that were fitted to obtain the averages shown in Figure 4B top are contained in the file Figure 4.xlsx

The fitting parameters and results for Figure 4B (top) are contained in the file Figure 4.xlsx

Figure 4B (middle):

The number matrices for the waveforms (calcium imaging and fit) shown in Figure 4b (middle) are contained in the file Figure 4.xlsx

The fitting parameters and results for Figure 4B (middle) are contained in the file Figure 4.xlsx

The original imaging data for this analysis is contained in movie S4

Figure 4B (bottom):

The number matrices for the waveforms (wingbeat frequency from events and fit) shown in Figure 4b (bottom) are contained in the file Figure 4.xlsx

The fitting results are contained in the file Figure 4.xlsx

Figure 4C:

The number matrices for the waveform shown in Figure 4C are contained in the file Figure 4.xlsx

The original calcium imaging movie as .avi file: DLM6_Imaging.avi

The original calcium imaging movie as tiff stack:  file DLM6_imaging_tiff

The fitting results are contained in the file Figure 4.xlsx

Figure 4E:

The number matrices for the waveforms shown in Figure 4E are contained in the file Figure 4.xlsx

The number matrices for the waveforms that resulted in the average waveforms shown in Figure 4E are contained in the file Figure 4.xlsx

The original data files for analyses in Figure 4E are contained as text files in the folders ShakB_oe (7 text files) and control (7 text files)