Differential expression signature of EpiCAM/CD45 positive cells in ascitic fluid from serous ovarian cancer patients

Differential transcriptome signature of FACS purified cell population (EpiCAM/CD45 dual positive cells vs EpiCAM only) from the ascites of human serous ovarian cancer. Primary objective: To characterize the phenotypic expression of stem cells markers, elucidation of differential gene expression and oncogenic functions in EpiCAM/CD45 dual positive vs EpiCAM cells. Methods: Cell Sorting (FACS) from ascites of human serous ovarian cancer patients, apoptotic assay, Drug sensitivity assay, tumor spheroid assay, Cell invasion and cell migration assay. Expression profiling of EpiCAM/CD45 dual positive and EpiCAM cells Platform: GPL10558; Catalog number : BD-103-0204; Array Content: HumanHT-12 v4 Expression BeadChip Kit; No. of Probes on Array: 47323; No.of Annotated Genes: 31000; No.of Control : 887; Label: Biotin; Molecule: RNA; No.of Sample sections on chip: 12