1Mutations generated were gene deletions, insertion of C-terminal His6 tags, nonsense mutations, or replacement of phage gene with gfp (Replace). 2Plaques recovered from electroporation were analyzed
An 8.1-kb fragment of the temperate Myxococcus xanthus phage Mx8 genome, when cloned into a plasmid vector, permits site-specific integration of the plasmid and confers superinfection immunity. Sequen
Phage 16-3 is a temperate phage of Rhizobium meliloti 41 which integrates its genome with high efficiency into the host chromosome by site-specific recombination through DNA sequences of attB and attP