Transcriptome analysis of immortalized OPLL ligament cells

Ossification of the posterior longitudinal ligament (OPLL) is formed by heterogeneous ossification of posterior longitudinal ligament. The patho-mechanism of OPLL is still largely unknown. For the rarity of the primary cell samples, here we immortalized the OPLL patient's posterior longitudinal ligament primary cells and normal patient's posterior longitudinal ligament primary cells. The transcriptome of the cells were compared and we found that the osteogenic ability of the OPLL ligament cells were inherited in the immortalized cells. mRNA profiles were done using primary cells of human ossification of the posterior longitudinal ligament (OPLL) tissue and normal posterior longitudinal ligament (PLL) tissue. The cells were immortalized using SV40 and hTERT insertion. The mRNA profiles of immortalized cells using either SV40 and hTERT insertion were also analyzed using sequencing technologies.