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资源简介：
CRISPR-SURF analysis of tiling CRISPR screen data
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创建时间：
2020-07-10
相关数据集
Multiple tissue sequencing of WT and Scx-ko mice
We used RNA sequencing and a gene knockout mice model to reveal the critical functions of Scx in multiple tissues. Overall design: Multiple tissues of Wild type(WT) and Scx-/- limb cells derived from
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The paired primers used for gene cloning, disruption, complement, silencing and expression.
*H = A, C or T; B = C, G or T; V = A, C or G; R = A or G. The enzyme sites are italicized while the gateway fragments for exchange are in bold.
Figshare2015-12-02 更新00
UHRF1 is a mediator of KRAS driven oncogenesis in lung adenocarcinoma [CRISPR_screen]. UHRF1 is a mediator of KRAS driven oncogenesis in lung adenocarcinoma [CRISPR_screen]
We performed a focused CRISPR in KRAS mutant lung cancer cells (A549) using a minipool sgRNA library targeting 80 lung cancer specific tumor suppressor genes and 12 control genes. This screen identifi
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Whole-genome CRISPR screen of etoposide-treated U2OS and U2OS p53KO cells, and RNAseq of U2OS cells incubated in media conditioned by etoposide-treated U2OS or U2OS p53KO cells.
CRISPR screen: U2OS or U2OS p53KO cells expressing Cas9 were transduced with a whole-genome library of CRISPR sgRNAs, then treated with either DMSO or etoposide. Differential sgRNA abundances were cal
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Active growth signalling promotes senescence and cancer cell sensitivity to CDK7 inhibition. Active growth signalling promotes senescence and cancer cell sensitivity to CDK7 inhibition
Tumour growth is driven by continued cellular growth and proliferation. Cyclin Dependent Kinase 7’s (CDK7) role in activating mitotic CDKs and globalgene expression makes it therefore an attractive ta
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