Single-cell dissection of human hematopoietic reconstitution after allogeneic hematopoietic stem cell transplantation

10,776 Lin-CD34+ cells and 317,677 total nucleated cells (TNCs) were captured by Single cell RNA sequencing from healthy control, transplant patients and their grafts. We serially profiled the transcriptome of transplanted HSPCs and TNCs to evaluate the hematopoietic process and regulatory mechanisms involved in reconstitution of HSPCs and their daughter cells post-HSCT. Notably, transcriptomic analysis enabled us to observe that immunoregulatory neutrophil progenitors (S100Ahigh Neus) were enriched in granulocyte colony-stimulating factor-mobilized peripheral blood stem cells. Single cell RNA sequencing were performed in BM and PB samples from 10 transplant patients and their G-CSF grafts. ***The raw data are stored in Genome Sequence Archive (GSA) with accession number HRA001359.***