Fate Specification Factors in Ad ARX Treated ARX ko hESCs.

(A) RT-qPCR of day 26 whole population samples examining the expression of three hormones and key transcription factors in ARX ko clone 1 cells treated with Ad GFP (green bars) or Ad ARX (blue bars) at an MOI of 2 on day 13 (filled bars) or 19 (open bars). * indicates p < 0.05 Ad GFP vs Ad ARX from a given transduction (Tdxn) day. N = 3 per group. (B) Immunostaining of PAX6 (green), somatostatin (red), and C-peptide (blue) in ARX ko cells differentiated to day 26 after transduction at day 13 or 19 with Ad GFP or Ad ARX. Inset is a ~3x enlarged portion from the region indicated by the star. Scale bar is 100 μm for all panels.