Microbial co-occurrence network reveals climate and geographic patterns for soil diversity on the planet

The Earth Microbiome Project has produced a variety of microbiome datasets that equally reflect various settings (EMPO level 3) and investigations conducted. These datasets are based on sequence data from the EMP database that have been corrected for errors and trimmed using Deblur in Qiime1. The complete workflow for soil collection, metadata curation, DNA extraction, sequencing, and sequence preparation, as well as the various subsets, can be retrieved from the EMP website (or FTP site). The complete quality filtered 90-bp BIOM table of release1 samples was used for the purposes of this study. Additionally, only samples that fit the EMPO3 ontology "Soil (non-saline)" were selected for analysis due to their simplicity. These samples were classified and labelled with climatic zones in line with the Köppen-Geiger climate classification system, based on the geographic coordinates of each location.