Results of SYBR Green real-time PCR on serial dilutions of each plasmids.
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Real-time PCR were performed with 2 µM of primers at Tm 50°C. Cells filled in bold indicate the biggest dilution for which both duplicate are PCR positive ( = limit of detection). ‘C’: number of plasmid copies use as template. ‘R’: PCR result, ‘+’: positive, ‘−’: negative.
创建时间：
2014-03-20
