Host gene expression in M. leprae-infected mouse FPs.
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aMice were infected with 3×107M. leprae. FP tissues were harvested and fixed in 70% ethanol on Day 1 and at 8 weeks post infection. RNA was purified using a FastPrep protocol, and cDNA was prepared from 1 µg RNA and subjected to PCR for TNF, IFNγ and CCL-2.bData was normalized to GAPDH and expressed as a log-fold increase over uninfected FPs.
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2015-12-02



