Breakdown of hardly degradable carbohydrates (lignocellulose) in a two-stage anaerobic digestion plant is favored in the main fermenter

Key questions: • Are hydrolysis fermenters useful for the biogas process? • How redundant are the different functions? • Does the function of MAGs differ in the different fermenters? • Which depencies exist (transporters vs. producers?), antimicrobial genes, antibiotic resistencies,phages-hosts? • Can we quantify the biomass turnover? • Can we find new pathways for SAO and Methanogenesis? • How active are the MAGs?