The RHOA-specific GTPase activating protein ARHGAP45 controls chemokine-driven migration of naïve T and B cells and of hematopoietic progenitors across blood endothelium

T cell recirculation and organ entry involve diapedesis through cytoskeleton remodeling. Part of this process is controlled by guanosine exchange factors (GEFs) and GTPase activating proteins (GAPs) that promote active and inactive forms of Rho family of GTPases. Among GAPs, we identified the ARHGAP45, also known as HMHA1 (human minor histocompatibility antigen 1) and determined its interactome by affinity purification coupled to quantitative mass spectrometry (AP-MS) in Jurkat cells. This dataset contains results of AP-MS of Jurkat cells expressing One-Strep-tagged (OST) ARHGAP45 prior to and after TCR stimulation. Each AP-MS purification of OST- protein is associated with a corresponding control (WT Jurkat) at the same time point of stimulation. For each time point, three independent biological replicates were performed and each biological replicate was analyzed in duplicate by mass spectrometry.