Minimum dataset for" Different cell imaging methods did not significantly improve immune cell image classification performance"

Minimum dataset for" Different cell imaging methods did not significantly improve immune cell image classification performance"88 images (4 channels (Alx594-conjugated anti-CD19 antibody fluorescence, BF, Ph, DIC) × 22 focal positions) were extracted from Experiment 1, and 91 images (3 channels (BF, Ph, DIC) × 30 time step + one Alx594-conjugated anti-CD19 antibody fluorescence) from Experiment 2. Since the images were extracted by dish, a total of 537 images (3 dish × 88 images for Experiment 1 and 3 dish × 91 images for Experiment 2). This is about 0.6% of the total number of images.File name in Experiment 1 is 180402_ils_lmpp-probmix_dish[dish number]_xy001c[channel]z[focan position].tifFile name in Experiment 2 is 180411_ils_lmpp-probmix_dish[dish number]_xy001c[channel]t[time step].tifchannel: '01':'Alx594-conjugated anti-CD19 antibody fluorescence', '02':'BF', '03':'Ph', '04':'DIC'

本研究选取了88张图像（包含4个通道，即Alx594偶联抗CD19抗体荧光、背向散射、偏振光、微分干涉对比度成像，每个通道对应22个焦点位置）作为实验1的最低数据集，以及91张图像（包含3个通道，即背向散射、偏振光、微分干涉对比度成像，每个通道对应30个时间步长，并附加一张Alx594偶联抗CD19抗体荧光图像）作为实验2的最低数据集。由于图像是从培养皿中提取的，因此总共获得537张图像（实验1为3个培养皿×88张图像，实验2为3个培养皿×91张图像），这大约占所有图像总数的0.6%。实验1中图像的文件名为180402_ils_lmpp-probmix_dish[培养皿编号]_xy001c[通道]z[焦点位置].tif，实验2中图像的文件名为180411_ils_lmpp-probmix_dish[培养皿编号]_xy001c[通道]t[时间步长].tif。其中，通道包括：'01'表示Alx594偶联抗CD19抗体荧光，'02'表示背向散射，'03'表示偏振光，'04'表示微分干涉对比度成像。