Four new genomes of the Pallas´s cat: an insight into the patterns of within-species variability

Manul (Otocolobus manul) is the only representative of the genus Otocolobus belonging to the Leopard Cat lineage. Their habitat is characterized by harsh environmental conditions. Although their populations are probably more stable than previously thought, it is still the case that their population size is declining. The main cause of their decline is the destruction of their natural habitat, which together with their natural behavior results in geographically fragmented populations and a potential threat of loss of genetic variability. Conservation programs exist to protect manuls, but those based on captive breeding are often unsuccessful due to their increased susceptibility to diseases. The manul is therefore a suitable model species for evolutionary and diversity studies as well as for studying mechanisms of adaptation to harsh environment and mechanisms of susceptibility to diseases. Whole genome sequencing (WGS) is an important tool for such studies, providing base-by-base view o..., Tissues of four different manul individuals (one female and three males) obtained from Czech ZOOs (ZOO Jihlava, ZOO Brno, ZOO Prague) were selected based on available information about their origin and relatedness. The samples were obtained either post-mortem or as part of veterinary procedures performed for other reasons.
DNA was extracted from available tissue samples (either blood, spleen or colon) using Qiagen (Germany) MagAttract HMW DNA isolation kit. The kit was used according to the manufacturer’s recommendations. Two isolations were made for each individual. DNA samples were evaluated in terms of purity (absorbance) and concentration using Tecan (Switzerland) Infinite 200 Pro plate reader. DNA samples were stored at 4°C for 5 days and then transported on dry ice to the Novogene sequencing facility in Germany. Samples were checked prior to library construction using Agilent 5400 fragment analyzer. All samples passed QC (quantity ≥ 200 ng; OD260/280=1.8-2.0, no degradation).
Sequ..., , # Four new genomes of the Pallas´s cat: an insight into the patterns of within-species variability

[https://doi.org/10.5061/dryad.pzgmsbcvt](https://doi.org/10.5061/dryad.pzgmsbcvt)

This dataset contains results of genome analyses performed on 4 individual genomes of Otocolobus manul. The files contained in this dataset are as provided by the sequencing facility (Novogene). This dataset serves as a full primary data repository for a peer-reviewed manuscript with the same name.

## Description of the data and file structure

**Report**

* *X204SC23111270-Z01-F002_Otocolobus_manul_Report*: *contains graphical overview of all collected statistical data in html format.
* *src*: structured elements used in the html report above (images, fonts, methods, etc.)

**Results**

contains subfolders of analysis results as presented in the graphical report above. Each subfolder contains readme file with further details.

* *01.OriginalData:* examples and explanation of input sequencing data

- *02....