Focused microarrays as a method to evaluate subtle changes in chicken embryonic heart gene expression. Gallus gallus

Microrrays plataforms with thousands of gene sequences have limited capacity to pick up subtle small changes in gene expression. A focused two-color microarray platform was designed for high replication and to use statistical power to detect small changes Keywords: time course, embryo development Overall design: Individual samples from 8 embryos were arraged on a complete interwoven loop design where each treatment (embryonic ages 7, 15, 19 and 20) was replicated 8 times (4 dye-swaps).