Hydrogen sulfide (H2S)-donating formyl peptide receptor 2 (FPR2) agonists: design, synthesis, and biological evaluation in primary mouse microglia culture

Chronic neuroinflammation and oxidative stress play an important role in the onset and progression of neurodegenerative disorders, including Alzheimer’s disease, which can ultimately lead to neuronal damage and loss. This study aimed to evaluate the anti-inflammatory and pro-resolving potential of novel hybrid FPR2 agonists with hydrogen sulfide (H₂S)-releasing properties in an in vitro model of neuroinflammation. The dataset comprises experimental data obtained from primary murine microglial cultures under basal conditions and following lipopolysaccharide (LPS) stimulation. The collection includes processed results from biochemical assays assessing inflammatory response and cell viability, including cytokine levels (ELISA), nitric oxide production (Griess assay), and cytotoxicity (LDH assay). The dataset enables analysis of the effects of tested compounds on microglial activation and inflammatory signaling. Both compounds (7b and 8b) were able to counterbalance LPS-induced cytotoxicity and the release of pro-inflammatory (IL-18, IL-6) and anti-inflammatory (IL-10) cytokines induced by LPS stimulation.