The transcriptomes in RGS10-depleted SKBR3 cells

RGS10 plays an important role in cell survival, polarization, adhesion, chemotaxis, and differentiation in various cancers. However, the mechanism underlying the function of RGS10 in breast cancer remains unclear. We compared the gene expression differences between the RGS silencing group and the wild group using RNA seq. The shRNA-RGS10 and shRNA negative control (NC) are transfected in SKBR3 cells. We utilized a series of functional experiments to verify the silencing of RGS10. The transcriptomes in RGS10-depleted SKBR3 cells and shRNA-NC SKBR3 cells were performed by second-generation high-throughput sequencing. This project measured a total of 6 samples using the BGISEQ platform, with an average output of 6.70G of data per sample. The average comparison rate of the sample compared to the genome is 90.38%, and the average comparison rate of the gene set is 70.96%; A total of 16606 genes were detected. By KEGG enrichment analysis, upregulated KEGG pathways were found to be associated ..., The dataset was collected by RNA sequencing. The raw sequencing data contains low-quality, contaminated joints, and reads with high levels of unknown base N. These reads need to be removed before data analysis to ensure the reliability of the results by SOAPnuke (v1.5.6). The high-quality reads were mapped and aligned to the human reference genome using HISAT2. The reference genome source is NCBI, and the reference genome version is GCF_000001405.39_GRCh38.p13. Alignment and quantification of reads were performed using Bowtie. The expression levels of genes were quantified to identify differentially expressed genes by RSEM (v1.3.1). To gain insight into the change of phenotype, GO (http://www.geneontology.org/) and KEGG (https://www.kegg.jp/) enrichment analysis of annotated different expression genes was performed by Phyper based on a Hypergeometric test. The data uploaded here has not undergone any processing.
RNA sequencing was performed by the BGI (Shenzhen, China). Briefly, RNA fro..., , # The transcriptomes in RGS10-depleted SKBR3 cells

Dataset DOI link: [https://doi.org/10.5061/dryad.7h44j102r](https://doi.org/10.5061/dryad.7h44j102r)

## Description of the data and file structure

Here are twelve groups of data for analysis. The group labeled shRNA-RGS10 represents breast cancer cell line SKBR3 after silencing the RGS10 gene. The shRNA-NC group serves as the negative control. Each set of sequencing was performed with three biological replicates. \_1 indicates the forward sequencing results, and \_2 indicates the reverse sequencing results. All data are presented in fastq format.

For a more detailed explanation:

* \"shRNA\" stands for short hairpin RNA, which is used to silence specific genes through RNA interference.
* \"RGS10\" refers to the Regulator of G protein Signaling 10, the gene that is being silenced.
* \"NC\" typically stands for \"Negative Control,\" meaning this group does not receive the gene silencing treatment.