Gene expression data of human chord blood hematopoietic progenior cells subjected to myeloid differentiation and transduced with lncRNA HOXA10-AS

Long non-coding RNAs (lncRNAs) have emerged as crucial regulators of gene expression and cell fate decisions. We predicted HOXA10-AS as a novel non-coding regulator of hematopoiesis and leukemogenesis We overexpressed HOXA10-AS using a bidirectional vector construct, which resulted in impaired monocytic in vitro differentiation and myeloid colony-formation. In addition, shRNA, sgRNA and LNA-GapmeR mediated downregulation of HOXA10-AS resulted in reduced leukemic growth of KMT2A-r cell lines and patient blasts. (p≤0.05). Here we assess early effects of ectopic HOXA10-AS expression in human HSPC subjected to myeloid differentiation conditions. Human CD34+ HSPC from cord blood were transduced with HOXA10-AS or a empty control vector. Transduced cells were subjected to myeloid differentiation conditions. CD11b+CD117-CD14- myeloid precursors were FACS sorted on day 5 of culture. We analyzed early gene expression changes upon ectopic HOXA10-AS expression using the Affymetrix Microarray Clariom™ S Pico Assay