Transcriptome analysis of transplanted vascular organoid grafts under cranial window at the time of thrombosis after SARS-CoV-2 spike-ECD infusion

We evaluated virus-induced damages in perfused human blood vessels from iPS-derived vascular organoid (iVO). By taking advantage of the cranial window model, iVOs were transplanted to develop human blood vessels by anastomosing the recipient mouse circulatory system. we infused SARS-CoV-2 spike protein (extracellular domain, ECD) in the mouse to determine the impact on chimeric human-mouse blood vessels formed under the cranial window. Our iVO transplantation studies indicated that Spike protein could specifically target human endothelial cells, but not murine endothelial cells, which leads to neutrophil migration by creating a coagulopathic milieu and therein generating microthrombi. Bulk RNA-seq of transplanted vascular organoid grafts under cranial window at day 1 after SARS-CoV-2 spike-ECD infusion.