hAEC are positive for naïve pluripotent markers.

(A) Representative micrographs at 20x from hAEC at P0-P2 immunostained for KLF4 (red) and TFE3 (red); the nuclei were stained with DAPI (blue). (B) Graph shows the percentage of hAEC that express naïve markers. (C) Representative micrographs at 20x from hAEC at P0-P2 immunostained for KLF4 (red) or TFE3 (red) and co-expressing TRA-1-60; the nuclei were stained with DAPI (blue). (D) Graph shows the percentage of cells immunostained for both TRA-1-60 and naïve markers. Results are expressed as percentages of means ± S.E.M. from 9 fields counted in duplicate from three independent experiments. Scale bar 50 μm. * p < 0.05 as compared with P0.