Supplementary date: Application of Gyrolab microfluidic platform to measure picomolar affinity of a PD-L1-binding Adnectin PET radioligand
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Supplementary Figure 1. Bioanalytical
characterization of Adnectin samples. (A) SDS-PAGE: lane 1, standard; lane 3,
His-ADX_5322_A02-DBCO; lane 5, ADX_5322_A02-DBCO; lane 6, 19F-BMS-986192;
lanes 2 and 4, other constructs. (B) Analytical SEC. (C) Mass spectrometry
(MS).
<b>Supplementary
Figure 2. </b>Multi-curve analysis of the affinity
series for PD-L1 Adnectins after 22 h or 44 h equilibration at room
temperature (data points and fitted curve: 50 pM Adnectin in orange and 150
pM Adnectin in blue). (A) ADX_5322_A02-DBCO, 44 h; (B) His-ADX_5322_A02-DBCO,
22 h; (C) His-ADX_5322_A02-DBCO, 44h; (D) <sup>19</sup>F-BMS-986192, 22 h; (E)
<sup>19</sup>F-BMS-986192, 44 h.
提供机构：
Taylor & Francis
创建时间：
2020-07-16
