Prevalence of PcaFV in Apostichopus californicus specimens collected in Southeast Alaska from Jun to Nov 2021

Understanding the prevalence and load of aquatic invertebrate flaviviruses is essential to estimating risk to fisheries. We surveyed the prevalence of the Apostichopus californicus (urn:lsid:marinespecies.org:taxname:529363) associated flavivirus (PcaFV) using two approaches: Loop-mediated isothermal amplification (LAMP) and quantitative reverse transcriptase PCR. We targeted this survey around populations of A. californicus: specimens collected from Sitka Sound, Sitka, Alaska (Baranof Island), Southeast Alaska, and Nanoose, British Columbia, Canada. We found that LAMP detected PcaFV in 21% of tested specimens, and qRT-PCR in 88% of tested specimens. The mean load of PcaFV in copies per ng RNA extracted was significantly higher in specimens that were positive by LAMP compared to those in which LAMP did not detect PcaFV. This dataset contains PcaFV prevalence determined by RT-LAMP and qRT-PCR, as well as associated collection metadata.