Table_4_Transcriptome Analysis of Multiple Metabolic Tissues in High-Salt Diet–Fed Mice.xlsx

High-salt diet (HSD) is associated with dysregulated metabolism and metabolic disorders. Although previous studies have indicated its effect on metabolic tissues, the involving molecular mechanisms are not quite understood. In the present study, we provided a comprehensive transcriptome analysis on multiple metabolic tissues of HSD-fed mouse model by RNA sequencing. We observed that several genes associated with de novo lipogenesis and cholesterol biosynthesis were significantly downregulated in white adipose tissue and liver tissue of HSD mice group, such as Fasn, Scd1, Acaca, and Thrsp. Furthermore, combined with secretome datasets, our results further demonstrated that HSD could alter expression levels of organokines in metabolic tissues, for example, Tsk and Manf, in liver tissue and, thus, possibly mediate cross-talk between different metabolic tissues. Our study provided new insight about molecular signatures of HSD on multiple metabolic tissues.

高盐饮食（HSD）与代谢失调及代谢性疾病密切相关。尽管既往研究已表明其对代谢组织的影响，但其所涉及的分子机制尚不明确。在本研究中，我们通过对高盐饮食喂养小鼠模型的多个代谢组织进行转录组分析，提供了全面的了解。研究结果显示，与去饱和脂肪酸合成和胆固醇生物合成相关的多个基因，如Fasn、Scd1、Acaca和Thrsp，在HSD小鼠组的白色脂肪组织和肝脏组织中显著下调。此外，结合分泌组数据集，我们的研究进一步揭示了HSD能够改变代谢组织中器官激肽的表达水平，例如在肝脏组织中Tsk和Manf的表达，从而可能介导不同代谢组织间的跨组织通讯。本研究为高盐饮食对多个代谢组织的作用的分子特征提供了新的见解。