TRuST: LINCS Backend Resource for Transcriptomic Signature-Driven Drug Repurposing

This dataset contains processed backend resources derived from the LINCS L1000 perturbational signature resource distributed through Bioconductor ExperimentHub (EH3226). The resource was prepared to support the deployment and reproducible use of TRuST (Transcriptomic Repurposing Signature web Tool), a Shiny-based framework developed for transcriptomic signature-driven drug repurposing analysis. The dataset includes an optimized HDF5-based signature matrix and associated metadata indexing files designed to enable efficient retrieval of LINCS transcriptomic signatures without requiring end users to download and preprocess the original resource locally. The backend preparation workflow involved retrieving the original LINCS resource through ExperimentHub, validating the underlying SummarizedExperiment object, extracting and cleaning the expression matrix, removing missing and duplicated gene identifiers, and generating optimized indexing files for rapid signature querying. The original LINCS L1000 data are publicly available through the LINCS consortium and Bioconductor resources. This Zenodo record contains only processed derivative resources generated for computational optimization, reproducible deployment, and integration within the TRuST framework. The original data ownership and licensing conditions remain with their respective providers.