Rachel Horowitz-Scherer, Chris Woodcock (2010) CIL:768, Patiria miniata, sperm. CIL. Dataset

Phosphorus has a characteristic electron energy loss with an edge at 160 eV. The Zeiss 902 electron microscope permits images to be recorded at different energy losses which can be digitized and processed to yield a net phosphorus image. For nuclear chromatin, phosphorus is primarily located in DNA, allowing its distribution in chromatin fibers to be mapped. Electron energy loss (EELS) imaging is also known as electron spectroscopic imaging (ESI) and electron energy filtering TEM (EFTEM).
Starfish sperm chromatin, which has clearly defined chromatin fibers was fixed, embedded in Lowicryl at low temperature, thin sections prepared and imaged unstained. The 3 grouped images were recorded with a Zeiss 902 energy filtered TEM at 80KV using 5eV windows centered on energy loss values of 170 (this image), 160, and 120eV.

磷元素具有特定的电子能量损失特征，其能量损失边缘位于160电子伏特。蔡司902型电子显微镜允许记录不同能量损失下的图像，这些图像可被数字化并处理，以获得净磷图像。对于核染色质，磷主要存在于DNA中，这使得其分布在染色质纤维中的分布得以绘制。电子能量损失成像（EELS）亦称为电子能谱成像（ESI）和电子能量过滤透射电子显微镜（EFTEM）。海星精子染色质，其染色质纤维界限清晰，经过低温固定、Lowicryl包埋后，制备了薄切片并进行无染色成像。三组图像使用蔡司902型能量过滤透射电子显微镜在80KV下，通过以170（此图像）、160和120电子伏特能量损失值为中心的5电子伏特窗口进行记录。