Determination of α1–6-fucosyltransferase activity.
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Recombinant FLAG-tagged Sf9 FUT8 was produced using the baculovirus-Sf9 cell system and purified using anti-FLAG M2 affinity gel. The fucosyltransferase activity of the recombinant protein was tested with GDP-[14C]-L-fucose, as substrate, after two days (2D) and three days (3D) of production. The transfer of [14C]-fucose is expressed in cpm.Determination of α1–6-fucosyltransferase activity.
创建时间:
2015-12-02



