Direct binding of BfmR to its own promoter.

A) EMSA showing that 6His-BfmR binds to its own promoter but not to the promoter of either rhlA or rhlC. B) Dye primer-based DNase I footprint assays show the protection pattern of the bfmR promoter after digestion with DNase I following incubation in the absence or presence of different amounts of 6His-BfmR, as indicated. Three BfmR-protected regions in the bfmR promoter DNA were demonstrated. C) bfmR promoter sequence with a summary of the DNase I footprint assay results. The BfmR-protected regions are underlined, and the putative BfmR box is in bold and in italics. Start codon of bfmR is in bold.