Bovine granulosa cells cultured on plastic dish or Xanthan and Locust been gum gel. Bos taurus strain:Japanese Black Cow

Granulosa cells are important for oocyte growth. When oocyte and granulosa cells (OGCs) derived from early antral follicles are cultured in vitro, they form antral like cavity and enclosed oocytes grow to the levels acquiring full developmental competence. Culture conditions of the OGCs profoundly affect this process such that using a polysaccharide gel made of Xanthan and Locust been gum as a culture substrate significantly stimulates growth of the oocytes and proliferation of the granulosa cells compared with those cultured on plastic substrate. This data is RNAseq of the granulosa cells of OGCs cultured for 14 days on plastic dish or gels. OGCs were collected form bovine early antral follicles (0.5-0.7 mm in diameter) of Japanese Black Cows. Ovaries were collected at a slaughterhouse and transported to the laboratory within 4 hours. At the end of culture period (16 days), the granulosa cells were collected from OGCs formed antrum and used for RNA seq. RNA in granulosa cells were extracted using RNAqueous (Invitrogen) and the RNA quality and concentration were examined using a Bioanalyzer (Agilent technologies, Palo Alto, CA, USA). Library quality and quantity were determined using the Agilent 2100 Bioanalyzer and the KAPA Library Quantification Kit (KAPA Biosystems, Wilmington, MA, USA), respectively. Clusters were generated on a cBot (Illumina), and one lane of the multiplied samples was sequenced as 75 bp reads (single read) on the NextSeq (Illumina). Image analysis, base calling and quality filtering were performed using the bcl2fastq2 v2.18.0.12 (Illumina) following the manufacturer's instructions. Sequence data were filtered to discard the adapter sequence, ambiguous nucleotides and low-quality sequences. The remaining sequence data were aligned to the Bos Taurus genome sequence (ARS-UCD1.2/bosTau9) to count sequence reads.