Fine Time-Course Expression Analysis of Mammalian Sex Determination in 129S1/SvImJ and C57BL/6J XX and XY mice

We analyzed the differentiation of the bipotential gonad into a testis or an ovary in 2 strains of mice - 129S1/SvImJ (129S1) and C57BL/6J (B6). Our results provide a high resolution view of the initiation and canalization of the differentiation of the gonad. It also reveals global differences in the transcriptome between 129S1 and B6 mice. Total RNA was obtained from XX and XY embryonic mouse gonads at 6 equally spaced time points between embryonic day (E) 11.0 and E12.0 from 129S1 and B6 mice. Mice were staged using tail somite numbers本研究针对129S1/SvImJ（简称129S1）与C57BL/6J（简称B6）两种小鼠品系，分析了双潜能性腺（bipotential gonad）向睾丸或卵巢的分化进程。本研究结果实现了对性腺分化起始与通道化过程的高分辨率解析，同时揭示了129S1与B6小鼠转录组的全局差异。我们从两类小鼠中，采集了胚胎日（embryonic day，E）11.0至E12.0之间6个等间隔时间点的XX、XY型胚胎小鼠性腺并提取总RNA；小鼠胚胎发育阶段通过尾体节数目进行分期。