Global gene expression analysis of vascular progenitors differentiated from human embryonic stem cells or induced pluripotent stem cells and their corresponding donor starting cells

Global gene expression analysis of CD31+ / CD146 +/- vascular progenitors (VP) derived from (a) human embryonic stem cells (VP-hESC), (b) adult fibroblast derived induced pluripotent stem cells (VP-AdF-iPSC), and (c) stromal primed cord blood CD34+ myeloid progenitor derived iPSC (VP-sp-CB-iPSC), (d) corresponding iPSC, and (e) corresponding starting fibroblasts and myeloid progenitors. Total RNA was harvested from (a) adult fibroblasts, (b) human myeloid progenitors: Non-nucloefected CD34+ CB cells that were expanded with growth factors from Day -3, and co-cultured with bone marrow stromal cells (BMSC) from Day 0 until Day+3. CD45+ myeloid progenitors were FACS-purified on Day +3 (c) FACS-purified CD31+ CD146 +/- vascular progenitors (VP) that were differentiated from human embryonic stem cells (hESC), (d) VP differentiated from low passage stromal-primed 7-factor episomal iPSC derived from growth-factor activated cord blood myeloid progenitors, and (e) VP differentiated from 4 factor viral construct iPSC derived from adult fibroblasts. Illumina HumanHT-12 V4.0 expression beadchips were used for all analyses in this series. Three independent samples of each condition were each run on individual microarrays.