Macrophages adopt mural cell function and marker expression for vascular support following ischemia. Macrophages adopt mural cell function and marker expression for vascular support following ischemia

We used scRNAseq to study if perivascular macrophages adopt a mural cell identity during tissue restoration in mouse models of ischemia Overall design: A total of 755 tdTomato+PDGFRβ+/- cells and 372 tdTomato-PDGFRβ+ cells from ischemic muscles at day 21 post-ischemia onset, and a total 190 tdTomato+PDGFRβ+/- cells and 192 tdTomato-PDGFRβ+ cells from healthy muscle were FACS sorted into Smart-seq2 plates in two batches, and processed using the Smart-seq2 protocol (Picelli et al., 2013). The single-cell transcriptomic sequencing was performed at Eukaryotic Single Cell Genomics Facility at SciLifeLab, Stockholm.