mRNA-Sequencing of two Aspergillis flavus strains with different levels of sclerotia fertility

Sclerotium (female) fertility, the ability of a strain to produce ascocarps in which meiosis occurs, influences internal morphological changes during sexual reproduction in Aspergillus flavus. Sclerotia from a high fertility cross (Hi-Fert-Mated), a low fertility cross (Lo-Fert-Mated), as well as from unmated strains (Hi-Fert-Unmated and Lo-Fert-Unmated) were each grown separately on mixed cereal agar and incubated at 30 *C in continuous darkness. Sclerotia were then harvested immediately after crosses were made and every two weeks until 8 weeks of incubation.

子座（雌性）的繁殖力，即菌株产生发生减数分裂的子囊果的能力，影响了黄曲霉菌在性繁殖过程中的内部形态变化。来自高繁殖力杂交（Hi-Fert-Mated）、低繁殖力杂交（Lo-Fert-Mated）以及未杂交菌株（Hi-Fert-Unmated 和 Lo-Fert-Unmated）的子座分别在不同的混合谷物琼脂上单独培养，并在30摄氏度的持续黑暗条件下进行培养。随后，在杂交完成后立即以及每隔两周直至培养8周后，子座被及时收获。