Differentially expressed messenger RNAs and long noncoding RNAs after spinal cord injury

Spinal cord injury (SCI) is a severely disastrous event that occurs in the central nervous system (CNS) that is associated with high disability and mortality rates.To further explore the expression of mRNAs and lncRNAs and the potential roles of differentially expressed mRNAs and lncRNAs after SCI, we examined the expression of mRNAs and lncRNAs in a rat model at 2 hours, 2 days and 7 days after SCI and identified the differentially expressed lncRNAs (DE lncRNAs) and differentially expressed mRNAs (DE mRNAs) using microarray analysis. A total of 992 mRNAs were differentially expressed at 2 hours after SCI, among which 730 mRNAs were up-regulated and 262 mRNAs were down-regulated. A total of 4308 mRNAs were differentially expressed at 2 days after SCI, including 2229 mRNA with up-regulated expression and 2079 mRNAs with down-regulated expression. A total of 4113 mRNAs were differentially expressed at 7 days after SCI, including 2339 up-regulated and 1774 down-regulated mRNAs. After SCI, 772 lncRNAs were differentially expressed in the 2-hour group (528 were up-regulated, 244 were downregulated) , 3193 lncRNAs were differentially expressed in the 2-day group (1332 were up-regulated, 1861 were downregulated) ,3093 lncRNAs were differentially expressed in the 7-day group (1290 were up-regulated, 1803 were downregulated) .The current study on provides novel insights into how lncRNAs and mRNAs regulate the pathogenesis of the immediate phase after SCI.脊髓损伤（spinal cord injury, SCI）是发生于中枢神经系统（central nervous system, CNS）的严重灾难性事件，伴随极高的致残率与死亡率。为进一步探究脊髓损伤后信使RNA（messenger RNA, mRNA）与长链非编码RNA（long non-coding RNA, lncRNA）的表达情况，以及差异表达的mRNA和lncRNA的潜在作用，本研究对脊髓损伤后2小时、2天及7天的大鼠模型中的mRNA与lncRNA表达水平进行了检测，并通过基因芯片分析鉴定出差异表达长链非编码RNA（differentially expressed lncRNAs, DE lncRNAs）与差异表达信使RNA（differentially expressed mRNAs, DE mRNAs）。脊髓损伤后2小时，共计992个mRNA呈现差异表达，其中730个上调、262个下调；损伤后2天，共计4308个mRNA呈现差异表达，包括2229个上调mRNA与2079个下调mRNA；损伤后7天，共计4113个mRNA呈现差异表达，其中2339个上调、1774个下调。脊髓损伤后，2小时组共计772个lncRNA呈现差异表达（528个上调，244个下调）；2天组共计3193个lncRNA呈现差异表达（1332个上调，1861个下调）；7天组共计3093个lncRNA呈现差异表达（1290个上调，1803个下调）。本研究为阐明长链非编码RNA与信使RNA调控脊髓损伤后急性期的发病机制提供了全新见解。