Gene expression profile of ARID3B knockout in HCT15 cells

Microarray analyses for identification of the differences in gene expression patterns have increased our understanding of the molecular mechanism of ARID3B in colon caner cellls.We used gene expression analysis data from HCT15-ARID3B-Cas9 and HCT15-Ctrl to identify differentially expressed probes. The Cas9/gRNA target sites containing20 bp gRNA sequence plus the PAM sequence (NGG) using Jack Lin’s CRISPR/Cas9 gRNA finder tool. The gRNA using for knockout ARID3B was predicted by blasting with human genomic and transcript sequences using the NCBI/blastn suite for the detection of potential off-targets.