Autism-Related Transcription Factors Underlying the Sex-Specific Effects of Prenatal Bisphenol A Exposure on Transcriptome-Interactome Profiles in the Offspring Prefrontal Cortex

We conducted experiments using the frontal cortex from neonatal pups prenatally exposed to BPA or vehicle control (BPA treament; pooled 3 pups frontal cortex, vehicle control; pooled 3 pups frontal cortex). 10-week-old female rats were daily treated with BPA at the concentration of 5,000 microgram/kilogram of maternal body weight or vehicle control from gestation day 1 (GD1) until parturition. Total RNAs were isolated and cDNAs were synthesized. The transcriptome profiles were performed using a high-throughtput RNA-seq.本研究采用产前暴露于双酚A（Bisphenol A，BPA）或溶剂对照的新生幼鼠前额叶皮层开展实验：实验组为3只幼鼠的前额叶皮层混合样本，溶剂对照组同样采用3只幼鼠的前额叶皮层混合样本。实验选取10周龄雌性大鼠，自妊娠第1天（GD1）起至分娩，每日按5000微克/千克母体体重的剂量给予BPA处理，溶剂对照组则给予等量溶剂。随后提取总RNA并合成互补脱氧核糖核酸（cDNA），利用高通量RNA测序（RNA-seq）技术获取转录组图谱。