Characterization of CD8+ virtual memory T cells in IL-4 knockout mice using single-cell RNA sequencing

We profiled the heterogeneity of virtual memory T cells and observed changes in virtual memory T cell subsets under conditions of IL-4 deficiency in mouse spleens using single-cell RNA sequencing. Using DEG analysis, we distinguished virtual memory T cells from splenocytes of wild-type B6 mice and IL-4 knock-out mice and compared virtual memory T cells of each condition.本研究表征了虚拟记忆T细胞（virtual memory T cells）的异质性，并采用单细胞RNA测序（single-cell RNA sequencing）技术，观测了小鼠脾脏内白细胞介素4（interleukin 4, IL-4）缺失条件下虚拟记忆T细胞亚群的变化。通过差异表达基因（differentially expressed genes, DEG）分析，我们将野生型B6小鼠与IL-4敲除小鼠的脾脏细胞中的虚拟记忆T细胞予以区分，并对两种实验条件下的虚拟记忆T细胞进行了比较分析。