scRNAseq of human iPSC-derived motor neurons from control and ALS patients.

We compare single cell transcriptomic profiles of motor neurons differentiated in vitro from human induced pluripotent stem cells derived from control and ALS patients. Overall design: Human induced pluripotent stem cell-derived motor neurons were differentiated in vitro over 18 days following a previously described protocol (Maury et al. Nat Biotechnol, 2015). Cells were processed for single cell sequencing following the manufacturer's guidelines for the 10X Genomics Chromium or Illumina/Bio-Rad ddSEQ platforms. A total of 25 samples were processed in 6 batches. Replicates of cell lines CS83iCTR-33n1, CS0179iCTR-n1, CS2XWCiALS-n1, CS29iALS-C9n1, CS52iALS-C9n6, CS25iCTR-18n2, and CS29iALS-C9n1.ISO were processed across multiple batches.