Efficiency of the versatile FAST vector assembly system.
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aCloning efficiency is based on 3 transformants for each assembled construct and calculated as the number of transformants that contained all inserts in correct orientation divided by the number of screened transformants.bAssembly of five double-stranded PCR fragments, and one oligonucleotide-based part;cReligation of the backbone vector fragment.
创建时间:
2015-12-02



