TGFb treated EpRAS cells undergoing hysteretic vs non-hysteretic EMT gene expression analysis.

EpRAS cells undergo EMT transition through hysteresis showing bistability of the system. The hysteresis transition is controlled by the negative feedback loop between miR-200s and Zeb1. Disruption of the feedback loop through the deletion of Z-box2 in the miR-200s cluster-2 promoter region changes the transition to unimodal without hysteresis. To determine the consequence of cells undergoing through hysteresis or unimodal transitions of EMT, herein we determined the transcriptomic profiles of EpRAS wild type and EpRAS-delZbox2 mutant cells, (designated as EpRAS-C2-2). To induce EMT, the cells were treated with 100pM TGFbeta for 3 days. Mock PBS treatment is used as control. 2x2 design. Comparison of two cells (EpRAS wild-type vs EpRAS mutant cells, in which the Z-box-2 of the miR-200c promoter is deleted and disrupts the negative feedback loop with Zeb1) and two treatment conditions, control and TGFb.