Wheat Enhanced Disease Resistance EMS-Mutants Include Lesion-mimics With Adult Plant Resistance to Stripe Rust

The datasets for 'Wheat Enhanced Disease Resistance EMS-Mutants Include Lesion-mimics With Adult Plant Resistance to Stripe Rust'. Main datasets combined.mapspart2.Lib20HetMinPer15HetMinCovVariableHomMinCovVariable.reformatted.corrected.10kb_bins.RH.byContig.MI.No_RH.maps.vcf combined.mapspart2.Lib20HetMinPer15HetMinCovVariableHomMinCovVariable.reformatted.corrected.10kb_bins.RH.byContig.MI.No_RH.maps.allGenes.snpeff.vcf combined.mapspart2.Lib20HetMinPer15HetMinCovVariableHomMinCovVariable.reformatted.corrected.10kb_bins.RH.byContig.MI.No_RH.maps.highConf.snpeff.vcf These three files contain substitutions called from the EMS mutant lines included in the study, as well as a non-mutagenized control line. For each line, different HetMinCov and HomMinCov were set to maximize the number of EMS-type mutations, while retaining % EMS mutations ≥ 98%. The selected parameters are recorded in our publication. The mutation effects were predicted with snpeff for either 69,909 high-confidence genes or both high- and low-confidence genes. The mutations that stemmed from residual heterogeneity were removed. Supplemental datasets combined.mapspart2.Lib20HetMinPer15HetMinCovVariableHomMinCovVariable.reformatted.corrected.10kb_bins.RH.byContig.MI.RH_only.maps.vcf combined.mapspart2.Lib20HetMinPer15HetMinCovVariableHomMinCovVariable.reformatted.corrected.10kb_bins.RH.byContig.MI.RH_only.maps.allGenes.snpeff.vcf combined.mapspart2.Lib20HetMinPer15HetMinCovVariableHomMinCovVariable.reformatted.corrected.10kb_bins.RH.byContig.MI.RH_only.maps.highConf.snpeff.vc These three files are produced in the same manner as the main datasets but contain substitutions that originate from residual heterogeneity. Variable_HetMinCov_HomMinCov_no_RH.zip This folder contains the raw output files of called mutations with varying HetMinCov and HomMinCov parameters, excluding mutations from residual heterogeneity. Variable_HetMinCov_HomMinCov_RH.zip This folder contains the raw output files of called mutations with varying HetMinCov and HomMinCov parameters for mutations related to residual heterogeneity. Orthogroups.zip Orthogroups-known_genes.zip This folder contains outputs from OrthoFinder that we used to infer orthology between known genes of interest and Kronos genes. Orthogroups.zip does not include known genes, while Orthogroups-known_genes.zip does.